Int. J. Dev. Biol. 42: 243 - 247 (1998)
© UPV/EHU Press

Creating mosaics in Drosophila.

N Perrimon

Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.

ABSTRACT The ability to create mosaic animals allows the phenotypic analysis of patches of groups of genetically different cells that develop in a wild type environment. In Drosophila, a variety of techniques have been developed over the years to generate mosaics, and in this chapter, I review the techniques that our laboratory has developed. These include the "Dominant Female Sterile" technique which allows the analysis of gene functions to oogenesis and embryogenesis; the "Gal4-UAS" technique which allows the control of where and when specific genes are expressed; and, the "Positive Marked Mutant Lineages" technique which allows clones of cells to express a specific reporter gene.