Int. J. Dev. Biol. 42: 243 - 247 (1998)

Vol 42, Issue 3

Special Issue: Developmental Genetics of Drosophila

Creating mosaics in Drosophila

Published: 1 April 1998

N Perrimon

Department of Genetics, Harvard Medical School, Boston, MA 02115, USA. perrimon@rascal.med.harvard.edu

Abstract

The ability to create mosaic animals allows the phenotypic analysis of patches of groups of genetically different cells that develop in a wild type environment. In Drosophila, a variety of techniques have been developed over the years to generate mosaics, and in this chapter, I review the techniques that our laboratory has developed. These include the "Dominant Female Sterile" technique which allows the analysis of gene functions to oogenesis and embryogenesis; the "Gal4-UAS" technique which allows the control of where and when specific genes are expressed; and, the "Positive Marked Mutant Lineages" technique which allows clones of cells to express a specific reporter gene.