Int. J. Dev. Biol. 40: 1061 - 104 (1996)

Vol 40, Issue 5

Long-term culture of avian embryonic cells in vitro

Published: 1 October 1996

T Kuwana, K Hashimoto, A Nakanishi, Y Yasuda, A Tajima and M Naito

Research Development Corporation of Japan, Kumamoto. kuwana@infobears.or.jp

Abstract

The pH of the embryonic blood, one of the most important environmental factors for embryonic cells, was found to range from 8.1 to 8.5 in chick embryos until 108 h after incubation. Based on these results, the culture medium adjusted to pH 8.0 was used to culture embryonic chick and quail cells. They were easily subcultured for a long period of time at pH 8.0. This pH culture condition may have wide application for manipulating embryonic cells or tissues and establishing cell lines from avian embryos.