Int. J. Dev. Biol. In Press
doi: 10.1387/ijdb.220002za
© UPV/EHU Press

Correlation between CDK1 protein and CDK1 mRNA during oocyte maturation in vivo in mouse

Sun Yating, Zhu Aizhen*

Reproductive Medicine department, Yuncheng Central Hospital of Shanxi Province, Yuncheng, Shanxi, China

ABSTRACT The aim of this study was to investigate correlation between CDK1 protein and CDK1 mRNA during oocytes maturation in vivo in mouse. GV, GVBD, MI and MII oocytes were obtained from mice respectively. Western blot validated that the CDK1 protein expression increased continuously and significantly with oocytes maturation in vivo (P<0.05). Real-time qRT-PCR showed that CDK1 mRNA expression was down-regulated significantly during transformation from GV to MI stages (P<0.05), and it was up-regulated significantly during transformation from MI to MII stages (P<0.05). The level of CDK1 mRNA reached the maximum at MII stages. Spearman correlation analysis indicated that CDK1 protein expression was poor correlation with CDK1 mRNA expression during oocytes maturation in vivo (R=0.200). This finding suggested that the increase of CDK1 protein during oocyte maturation in vivo was not entirely caused by the change of transcription level. The results provide new thought for further research on the molecular mechanism of oocyte maturation in vivo.

Keywords:

mouse, oocyte maturation in vitro (IVM), maturation-promoting factor (MPF), cyclin dependent kinases 1 (CDK1), germinal vesicle breakdown (GVBD)

*Corresponding author e-mail: zhuaizhen8768@163.com