Int. J. Dev. Biol. In Press
doi: 10.1387/ijdb.210151dr
© UPV/EHU Press

Spatial distribution of blood vessels in the chick embryo chorioallantoic membrane

Diego Guidolin1, Roberto Tamma2, Tiziana Annese2, Cinzia Tortorella1 and Domenico Ribatti2

1Department of Neuroscience, Section of Anatomy, University of Padova, Padova and 2Department of Basic Medical Sciences, Neurosciences, and Sensory Organs, University of Bari Medical School, Bari, Italy

ABSTRACT The chick embryo chorioallantoic membrane (CAM) is a useful tool to study both angiogenesis and anti-angiogenesis in vivo. CAM vascular growth pattern including the way through vessels fills the available space can be pretty easily described and quantified using image analysis procedures, in order to evaluate different parameters, including fractal dimension, lacunarity and non-fractal order-disorder parameters. In the present study, we further expanded this morphological description, by estimating an index expressing the degree of symmetry characterizing the CAM vascular tree structure in the course of the embryonic development. Moreover, a uniformity index was estimated to quantitatively characterize the space-filling features of the vessels, i.e. the degree of spatial uniformity of their distribution in the tissue.


Angiogenesis, chorioallantoic membrane, spatial distribution vascular growth, vascular morphogenesis

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