Int. J. Dev. Biol. In Press
doi: 10.1387/ijdb.160026lh
© UPV/EHU Press

Genetic Transformation of Fungi

Liya He, Jiao Feng, Sha Lu, Zhiwen Chen, Chunmei Chen, Ya He, Xiuwen Yi, and Liyan Xi*

Department of Dermatology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China

ABSTRACT Transferring DNA into cells is an essential method of research on molecular cloning and gene function. As the molecular biology and materials physics develop, more and more new transformation methods have been applied in mammalian cells. Some techniques have been successfully developed for several types of fungi, but their efficiencies are extremely low. To better study on functional genes of fungi, and to improve the characteristics of fungi in an easy, safe and reliable way, many research are made to effectively develop such technologies in a wide variety of species and to increase the efficiency and reproducibility of genetic transformation. The objective of this paper is to review the latest development of transformation methods used for the genetic transformation of fungi, mainly including several promising transformation approaches and their advantages and drawbacks, which may open up a new way for research in fungi.


Genetic Transformation, Fungi, Electroporation, Liposome, Nanomaterial

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