Int. J. Dev. Biol. 57: 517 - 523 (2013)

https://doi.org/10.1387/ijdb.130176mm

Vol 57, Issue 6-7-8

Special Issue: Plant Transgenesis

High-throughput analysis of rice genes by means of the heterologous full-length cDNA overexpressor (FOX)-hunting system

Review | Published: 30 August 2013

Mieko Higuchi-Takeuchi¹, Masaki Mori² and Minami Matsui*^,1,3

¹RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan, ²Disease Resistance Crops Research and Development Unit, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan and ³Biomass Engineering Program Cooperation Division, RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan

Abstract

Mutant populations are indispensable tools for investigating plant gene functions. Gain-of-function technology is one of the approaches used for the systematic production of mutant resources and activation tagging is a well-established method to generate gain-of-function mutants in plants. As an alternative approach for the systematic generation of a gain-of-function mutant population, we developed the Full-length cDNA OvereXpressor (FOX)-hunting system in which full-length cDNAs (fl-cDNAs) are overexpressed in plants to quickly identify candidate genes. The FOX-hunting system was used for high-throughput analysis of rice (Oryza sativa) genes heterologously expressed in Arabidopsis thaliana (rice FOX Arabidopsis lines). A large screening to identify and characterize rice genes with rice FOX Arabidopsis lines revealed that one of the isolated genes, BROAD-SPECTRUM RESISTANCE 1 (BSR1) conferred multiple or broad-spectrum disease resistance in both a dicotyledonous and monocotyledonous plant. We found that expression of rice fl-cDNAs without a homolog in Arabidopsis affected morphological traits. In addition, overexpression of homologous genes of rice and Arabidopsis led to a similar phenotype. Thus, we conclude that the FOX-hunting system is an excellent heterologous system and offers a new tool with which to explore gene function in rice.

Keywords

full-length cDNA, rice, Arabidopsis, gain-of-function