Int. J. Dev. Biol. 46: 741 - 745 (2002)
© UPV/EHU Press

Developmental potential of mouse tetraploid cells in diploid <--> tetraploid chimeric embryos.

Yuji Goto, Junko Matsui and Nobuo Takagi

Division of Bioscience, Graduate School of Environmental Earth Science and Research Center for Molecular Genetics, Hokkaido University, Japan.

ABSTRACT Mouse 2n (lacZ-) <--> 4n (lacZ+) aggregation chimeras were examined 5 or 10 days after uterine transfer to test the potential of 4n cells to contribute to embryonic tissues. Recovered embryos corresponded to embryonic day 7.5 approximately 8.0 and 12.5, respectively. Ten days after transfer, 4n cells were never detected, as reported earlier, in embryonic tissues of chimeras produced by the standard procedure in which one 2n embryo at the8-cell stage is aggregated with a4n embryo at the4-cell stage. However, beta-gal positive cells were present in embryonic tissues, though in a low number, in chimeras produced by a 2n and a 4n embryo at the 4-cell stage. Similar results were obtained when one 2n embryo atthe 8-cell stage was aggregated with two 4n embryos atthe 4-cell stage. beta-gal positive cells were found in the heart, liver, skin and intestinal epithelium. The majority of chimeras 5 days after uterine transfer retained beta-gal positive cells in embryonic tissues. The complete lack of 4n cell contribution to chimeras produced by the standard procedure is therefore attributed to the initial low proportion of 4n cells allocated to epiblast and their severe elimination from embryonic tissues.