Int. J. Dev. Biol. 45: 619 - 622 (2001)

Vol 45, Issue 3

Special Issue: Mammalian Reproduction and Development

In situ hybridization to mRNA: from black art to guiding light

Published: 1 May 2001

P Koopman

Institute for Molecular Bioscience, The University of Queensland, Brisbane, Australia. p.koopman@imb.uq.edu.au

Abstract

In situ hybridization to mRNA in embryo sections or wholemount embryos is one of the most powerful analytical tools available to the molecular developmental biologist. For many workers, this procedure provides the first insights into the function of newly isolated genes, allowing the formulation of hypotheses and setting the course for further research. This paper presents a personal historical perspective of the development of in situ hybridization, looks at the theory and practice of the technique, summarizes the current state of the art, and speculates on possible directions for the future as a tool in functional genomics.