Int. J. Dev. Biol. 60: 237 - 243 (2016)
doi: 10.1387/ijdb.160125bb
© UPV/EHU Press

Escherichia coli-based cell-free extract development for protein-based cancer therapeutic production

Amin S.M. Salehi, Conner C. Earl, Christina Muhlestein and Bradley C. Bundy*

Department of Chemical Engineering, Brigham Young University, Provo, UT, USA

ABSTRACT Cell-free protein synthesis has been around for decades but it has never been close to becoming a robust tool for the production of biotherapeutic agents. In this review, we focus on how Escherichia coli-based cell-free protein synthesis can be modified in various ways to produce challenging, complex anticancer biotherapeutics. Here we report progress in extract preparation and its relation to cell-free cancer research. The future prospects of cell-free technology and its potential in various areas of cancer therapeutics production are also highlighted.

Keywords:

cell-free protein synthesis, cell extract, cancer research, anticancer biotherapeutics

*Corresponding author e-mail: bundy@byu.edu