Int. J. Dev. Biol. 51: 723 - 729 (2007)
doi: 10.1387/ijdb.072352ns
© UPV/EHU Press

An efficient method for isolation of murine bone marrow mesenchymal stem cells

Samad Nadri1,2, Masoud Soleimani*,3, Reza H. HosSeni2, Mohammad Massumi4, Amir Atashi1,3 and Reza Izadpanah5,6

1Stem Cells and Tissue Engineering Department, Stem Cell Technology, Tehran, Iran, 2Biochemistry Department, Faculty of Science, Payam Noor University Unit Tehran, Tehran, Iran, 3Hematology Department, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran, 4Institute of Biochemistry and Biophysics Dept. Biochemistry, Tehran University, Tehran, Iran, 5Department of Pharmacology, Tulane University Health Science Center, New Orleans, LA, USA and 6Division of Gene Therapy, Tulane National Primate Research Center, Tulane University Health Sciences Center, Covington, LA, USA

ABSTRACT Mesenchymal stem cells (MSCs) have been isolated based on the ability of adherence to plastic surfaces. The potential of these cells to differentiate along multiple lineages is the key to identifying stem cell populations in the absence of molecular markers. Here we describe a homogenous population of MSCs from mouse bone marrow isolated using a relatively straightforward and novel approach. This method is based on the combination of frequent medium change (FMC) and treatment of the primary cultures with trypsin. Cells isolated using this method demonstrated the MSCs characteristics including their ability to differentiate into mesenchymal lineages. MSCs retained the differentiation potentials in expanded cultures up to 10 passages. Isolated MSCs were reactive to the CD44, Sca-1, and CD90 cell surface markers. MSCs were negative for the hematopoietic surface markers such as CD34, CD11b, CD45, CD31, CD106, CD117 and CD135. The data presented in this report indicated that this method can result in efficient isolation of homogenous populations of MSCs from mouse bone marrow.

Keywords:

murine, bone marrow, mesenchymal stem cell, isolation

*Corresponding author e-mail: soleim_m@modares.ac.ir