Vol. 63 Nos. 6/7 (2019) pp.259-316

Cover legend

In this issue, Sosa et al. used bisected Xenopus laevis embryos to analyze genome-wide transcriptional regulation during twinning. The center image shows a normal tailbud whole embryo, which displays a dorsal crescent (D) and a more pigmented ventral (V) region at midblastula. In the left and right sagittal half embryos identical twins are generated. Note that the distribution of maternal pigment is asymmetric due to a 90° rotation of the Spemann-Mangold organizer center in these identical twins. Dorsal half embryos scale to form a well-patterned embryo, whereas ventral half embryos lack a dorsal axis and consist of only ventral tissues with a large coelomic cavity. The data presented represents a treasure trove of gene regulation events during early embryo regeneration. For further information, see Sosa et al., pp. 301-309.

Original Articles

Apoptosis in Hydra: function of HyBcl-2 like 4 and proteins of the transmembrane BAX inhibitor motif (TMBIM) containing family
Mina Motamedi, Laura Lindenthal, Anita Wagner, Margherita Kemper, Jasmin Moneer, Mona Steichele, Alexander Klimovich, Jörg Wittlieb, Marcell Jenewein and Angelika Böttger
Int. J. Dev. Biol. (2019) 63: 259-270
Definitive endoderm differentiation is promoted in suspension cultured human iPS-derived spheroids more than in adherent cells
Shigeharu G. Yabe, Junko Nishida, Satsuki Fukuda, Fujie Takeda, Kiyoko Nashiro, Masato Ibuki and Hitoshi Okochi
Int. J. Dev. Biol. (2019) 63: 271-280

Technical Articles

Advanced microinjection protocol for gene manipulation using the model newt Pleurodeles waltl
Toshinori Hayashi, Mie Nakajima, Mitsuki Kyakuno, Kanako Doi, Ikumi Manabe, Shouhei Azuma and Takashi Takeuchi
Int. J. Dev. Biol. (2019) 63: 281-286
Use of soluble sperm extract to improve cloning efficiency in zebrafish
Sukumal Prukudom, Gloria I. Perez, Jose B. Cibelli and Kannika Siripattarapravat
Int. J. Dev. Biol. (2019) 63: 287-293
Isolation and phenotyping of potential stem cells from the umbilical cord of the bottlenose dolphin(Tursiops truncatus)
Annalaura Mancia, Giulia Zuccon, Denise Lunardi, Barbara Biancani, Claudia Gili, Roscoe Stanyon, Daniel García-Parraga, Mara Mellado, Luigi Abelli and Victoria Moreno-Manzano
Int. J. Dev. Biol. (2019) 63: 295-299

Developmental Expression Pattern

Transcriptome analysis of regeneration during Xenopus laevis experimental twinning
Eric A. Sosa, Yuki Moriyama, Yi Ding, Nydia Tejeda-Muñoz, Gabriele Colozza and Edward M. De Robertis
Int. J. Dev. Biol. (2019) 63: 301-309
Comparative analysis of p4ha1 and p4ha2 expression during Xenopus laevis development
Davide Martini, Martina Giannaccini, Viviana Guadagni, Silvia Marracci, Guido Giudetti and Massimiliano Andreazzoli
Int. J. Dev. Biol. (2019) 63: 311-316

The International Journal of Developmental Biology
ISSN 1696-3547 (online) and 0214-6282 (print)