Vol. 60 Nos. 7/8/9 (2016) pp.189-320

Cell-free Extracts in Development & Cancer Research

Cover legend

Cell free-extract production from Xenopus laevis eggs or embryos in a nutshell. Inserts on the left, from the top to the bottom: a Xenopus laevis female with eggs laid overnight; harvested eggs in a beaker; and three layers obtained after the first centrifugation (lipids on the top, cytoplasmic extract in the intermediate fraction and the pigment, DNA and cellular debris at the bottom). The main image shows the final step of cell-free extract purification: the clear extract is separated from leftover pigment after the final centrifugation. Photos by Mikolaj Cup (MISMaP, University of Warsaw, Poland and IGDR, University Rennes 1, France).

Preface

Cell-free extracts in Development and Cancer Research for over 40 years
Jacek Z. Kubiak
Int. J. Dev. Biol. (2016) 60: 189-191

Interview

MPF, starfish oocyte and cell-free extract in the background - an interview with Takeo Kishimoto
Jacek Z. Kubiak and Takeo Kishimoto
Int. J. Dev. Biol. (2016) 60: 193-200

Introductory Papers

Xenopus cell-free extracts and their contribution to the study of DNA replication and other complex biological processes
J. Julian Blow and Ronald A. Laskey
Int. J. Dev. Biol. (2016) 60: 201-207 A sea urchin cell-free system to study male pronuclear assembly and activation
Dominic Poccia
Int. J. Dev. Biol. (2016) 60: 209-219 Studying essential DNA metabolism proteins in Xenopus egg extract
Vincenzo Sannino, Arun M. Kolinjivadi, Giorgio Baldi and Vincenzo Costanzo
Int. J. Dev. Biol. (2016) 60: 221-227

Review Articles

Cell-free Xenopus egg extracts for studying DNA damage response pathways
Steven Cupello, Christine Richardson and Shan Yan
Int. J. Dev. Biol. (2016) 60: 229-236 Escherichia coli-based cell-free extract development for protein-based cancer therapeutic production
Amin S.M. Salehi, Conner C. Earl, Christina Muhlestein and Bradley C. Bundy
Int. J. Dev. Biol. (2016) 60: 237-243 The master Greatwall kinase, a critical regulator of mitosis and meiosis
Suzanne Vigneron, Perle Robert, Khaled Hached, Lena Sundermann, Sophie Charrasse, Jean-Claude Labbé, Anna Castro and Thierry Lorca
Int. J. Dev. Biol. (2016) 60: 245-254 Aurora-A: an expedition to the pole of the spindle in Xenopus egg extracts
Jacek Z. Kubiak and Claude Prigent
Int. J. Dev. Biol. (2016) 60: 255-261 Ubiquitin-mediated proteolysis in Xenopus extract
Gary S. McDowell and Anna Philpott
Int. J. Dev. Biol. (2016) 60: 263-270 Chaperone-mediated chromatin assembly and transcriptional regulation in Xenopus laevis
Takashi Onikubo and David Shechter
Int. J. Dev. Biol. (2016) 60: 271-276 Use of Xenopus cell-free extracts to study size regulation of subcellular structures
Predrag Jevtić, Ana Milunović-Jevtić, Matthew R. Dilsaver, Jesse C. Gatlin and Daniel L. Levy
Int. J. Dev. Biol. (2016) 60: 277-288 Reprogramming of somatic cells and nuclei by Xenopus oocyte and egg extracts
Alexander A. Tokmakov, Tetsushi Iwasaki, Ken-Ichi Sato and Shinji Kamada
Int. J. Dev. Biol. (2016) 60: 289-296

Original Articles

Single-molecule, antibody-free fluorescent visualisation of replication tracts along barcoded DNA molecules
Francesco De Carli, Vincent Gaggioli, Gaël A. Millot and Olivier Hyrien
Int. J. Dev. Biol. (2016) 60: 297-304 Flexibility vs. robustness in cell cycle regulation of timing of M-phase entry in Xenopus laevis embryo cell-free extract
Mateusz Debowski, Mohammed El Dika, Jacek Malejczyk, Robert Zdanowski, Claude Prigent, Jean-Pierre Tassan, Malgorzata Kloc, Miroslaw Lachowicz and Jacek Z. Kubiak
Int. J. Dev. Biol. (2016) 60: 305-314 Chromatin assembly and transcriptional cross-talk in Xenopus laevis oocyte and egg extracts
Wei-Lin Wang and David Shechter
Int. J. Dev. Biol. (2016) 60: 315-320

The International Journal of Developmental Biology
ISSN 1696-3547 (online) and 0214-6282 (print)