Transplantation of testis germinal cells into mouse seminiferous tubules. The film strip shows in sequence the technique for microinject ing cells using two procedures: rete testis and collecting duct injection. In the background un der the film strip are shown microscopic cross sections of mouse seminiferous tubules. From top to bottom on the left: the top panel shows normal mouse spermatogenesis, which is mostly obscu red by the film strip; in the second panel, seminiferous tubules of a busulfan-treated mouse us ed as a transplant recipient are shown. Note Sertoli cells on basement membrane and absence of germ cells; in the third frame are seen seminiferous tubules of a mutant Wv/Wv recipient mouse which contain only Sertoli cells and lack endogenous germ cells. From top to bottom on the righ t: first panel is a seminiferous tubule of a busulfan-treated recipient 110 days following tran splantation of transgenic testis cells that can be stained blue. The blue cells indicate that t he spermatogenesis is from transplanted transgenic donor cells; second panel on right is a semi niferous tubule showing spermatogenesis (blue cells) from donor spermatogonial stem cells that had been frozen, stored and then thawed before transplantation; third panel on right shows the edge of a seminiferous tubule of a recipient immunodeficient mouse into which transgenic rat te stis cells had been transplanted. The blue cells indicate the presence of rat spermatogenesis w hich produces normal rat spermatozoa that can be found in the mouse epididymis. For details see "Transplantation of testis germinal cells into mouse seminiferous tubules" by Ogawa et al. in this issue.

Contents

REVIEW

Development and regeneration of muscle spindles in mammals and birds
by Alfred Maier

ORIGINAL ARTICLES

Molecular cloning of Xenopus hatching enzyme and its specific expression in hatching gland cells
by Chiaki Katagiri, Ryu Maeda, Chiaki Yamashika, Koichi Mita, Thomas D. Sargent and Shigeki Yasumasu

Timing of the expression of enamel gene products during mouse tooth development
by Margarita Zeichner-David, hang Vo, henry Tan, Thomas diekwisch, Brett Berman, Flavia Thiemann, M. Dolores Alcocer, Patty Hsu, Tracy Wang, Julia Reyna, Javier Caton, Harold C. Slavkin and Mary MacDougall

Characterization of monoclonal antibodies againts tenascin-C: no apparent effect on kidney development in vitro
by Jan F. Talts, Hubert Eng, Hong-Yan Zhang, Andreas Faissner and Peter Ekblom

X-chromosome inactivation during the development of the male urogenital ridge of the mouse
by Robyn V. Jamieson, Sheila X. Zhou, Seong-Seng Tan and Patrick P.L. Tam

Neural induction and patterning in embryos deficient in FGF signaling
by Susan Godsave and Anthony J. Durston

The in vivo and in vitro effects of bone morphogenetic protein-2 on the development of the chick mandible
by Sunetra Ekanayake and Brian K. Hall

Sequential synthesis of cartilage and bone marker proteins during transdifferentiation of mouse Meckel's cartilage chondrocytes in vitro
by Kiyoto Ishizeki, Yuji Hiraki, Miyayuki Kubo and Tokio Nawa

Differential effects of transforming growth factors ß1, ß2, ß3 and ß5 on chondrogenesis in mouse limb bud mesenchymal cells
by Jesús Chimal-Monroy and Lino Díaz de León

Hydrocortisone modulates the expression of c-ets-1 and 72 kDa type IV collagenase in chicken dermis during early feather morphogenesis
by Nathalie Turque, Giovanna Buttice, Anne Beuscart, Dominique Stehelin, Pascale Crepieux and Xavier Desbiens

TECHNICAL ARTICLE

Transplantation of testis germinal cells into mouse seminiferous tubules
by Takehiko Ogawa, Juan Arechaga, Mary R. Avarbock and Ralph L. Brinster

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International Journal of Developmental Biology