TY  - JOUR
TI  - Stage-specific gene expression during rat spermatogenesis: application of the mRNA differential display method.
AU  - Geisinger, A
AU  - Wettstein, R
AU  - Benavente, R
T2  - The International Journal of Developmental Biology
AB  - Spermatogenesis is a complex differentiation process which requires the coordinate synthesis of diverse stage-specific proteins. In attempting a large-scale identification and characterization of those proteins, we have made use of the recently described mRNA differential display method (Liang and Pardee, Science 257: 967-971, 1992). This method is based on the reverse transcription of mRNAs obtained from two different cell populations (pachytene spermatocytes and spermatids in the present study) followed by a PCR reaction and comparison of the individual cDNA populations in a polyacrylamide gel system. Up to the present we have been able to identify 268 cDNA bands. Most of them (77%) are common to both cell stages. From the differentially expressed bands (23%) an ample majority was spermatid-specific (74%). According to our present results we conclude that the mRNA differential display is a promising approach for investigations on stage-specific gene expression during a differentiation process like spermatogenesis.
PY  - 1996
VL  - 40
IS  - 1
SP  - 385
EP  - 388
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/8735952
Y2  - 2024/05/21/07:31:07
ER  -