TY  - JOUR
TI  - Synthesis and secretion of a 38-kDa glycopolypeptide coincides with L6 myoblast fusion.
AU  - Metsikko, K
AU  - Vaananen, H K
T2  - The International Journal of Developmental Biology
AB  - Rat L6 myoblastic cell line fused rapidly after two day cultivation in a medium containing horse serum and insulin. We analyzed whether the induction of plasma membrane or secreted proteins occurred simultaneously with ongoing fusion. Thus the cells were metabolically labeled with [35S]methionine followed by biotinylation of the cell surface proteins. Detergent-solubilized proteins derivatized with biotin were isolated with streptavidin-agarose and subjected to SDS polyacrylamide gel electrophoresis. This analysis did not show fusion-associated induction of any surface proteins. However, analysis of the microsomal fraction revealed a fusion-associated 38-kDa glycopolypeptide. This polypeptide appeared simultaneously with the formation of the multinucleated cells and then declined with decreasing fusion activity. Pulse-chase labeling experiments showed that the 38-kDa component was secreted into the medium. These results indicate that a secreted protein component is induced during the fusion of L6 myoblasts.
PY  - 1993
VL  - 37
IS  - 2
SP  - 305
EP  - 310
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/8398677
Y2  - 2024/05/05/01:16:41
ER  -