TY  - JOUR
TI  - Full activation of the rat oocyte by protein synthesis inhibition requires protein phosphatase activity.
AU  - Zernicka-Goetz, M
AU  - Weber, M
AU  - Maro, B
T2  - The International Journal of Developmental Biology
AB  - The rat oocyte provides an interesting system in which to dissect the control mechanisms involved in the transition between a meiotic M phase and a mitotic interphase. In this study, we show that in rat oocytes activated parthenogenetically by puromycin, okadaic acid (a potent inhibitor of protein phosphatases 1 and 2A) induced an increase in histone H1 kinase activity suggesting that MPF was reactivated. However, the inhibition of phosphatases 1 and 2A shortly after second polar body extrusion did not allow the formation of a metaphase-like spindle, although microtubule polymerization was not inhibited. Instead, the chromatin remained condensed as a single mass and a large aster formed around it.
PY  - 1993
VL  - 37
IS  - 2
SP  - 273
EP  - 277
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/8398674
Y2  - 2024/05/18/19:39:05
ER  -