TY  - JOUR
TI  - Plant protoplasts as genetic tool: selectable markers for developmental studies.
AU  - Negrutiu, I
AU  - Hinnisdaels, S
AU  - Cammaerts, D
AU  - Cherdshewasart, W
AU  - Gharti-Chhetri, G
AU  - Jacobs, M
T2  - The International Journal of Developmental Biology
AB  - Protoplasts have usually been presented as a methodological tool. Even as such, they make possible an impressive array of applications in plant biology. Here we report on the use of protoplast-derived selectable markers in the study of several disturbed genetic systems with obvious effects on plant development: (1) auxotrophic mutants and the control of amino acid biosynthesis and transport in vegetative and reproductive tissues; (2) introgression of alien genetic information across phylogenetic boundaries by protoplast fusion, a consequence of controlled dedifferentiation-redifferentiation processes and attenuated incompatibility reactions in cultured cells; (3) expression (in)stability of foreign genes in transgenic plants during successive meiotic generations and in crosses between independent transformants.
PY  - 1992
VL  - 36
IS  - 1
SP  - 73
EP  - 84
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/1627478
Y2  - 2024/05/04/13:55:00
ER  -