TY  - JOUR
TI  - Autonomous mesoderm formation in blastocoelic roof explants from inverted Xenopus embryos.
AU  - Tencer, R
AU  - Goldfinger, M
T2  - The International Journal of Developmental Biology
AB  - Xenopus eggs, artificially fertilized, were prevented from undergoing equilibrium rotation by incubation in medium containing ficoll. Three orientations were selected: normal, with animal pole uppermost; inverted, with vegetal pole away from gravity; and an off-axis orientation, with embryos tilted approximately 90 degrees from the animal-vegetal axis. At blastula stage 8, cells forming the blastocoelic roof were cultured in isolation as explants. These cells are normally fated to from epidermis ventrally and neural derivatives dorsally. Unexpectedly, in the fragments originating from inverted or 90 degrees-off-axis embryos, axial structures were found: notochord, somites, neural cells, cement glands, and sometimes sensory organs. Inverted eggs could be exploited in studies of mesodermal specification.
PY  - 1992
VL  - 36
IS  - 1
SP  - 115
EP  - 122
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/1627462
Y2  - 2024/04/29/03:51:38
ER  -