TY  - JOUR
TI  - A simple method of image analysis to estimate CAM vascularization by APERIO ImageScope software
AU  - Marinaccio, Christian
AU  - Ribatti, Domenico
T2  - The International Journal of Developmental Biology
AB  - The chick chorioallantoic membrane (CAM) assay is a well-established method to test the angiogenic stimulation or inhibition induced by molecules and cells administered onto the CAM. The quantification of blood vessels in the CAM assay relies on a semi-manual image analysis approach which can be time consuming when considering large experimental groups. Therefore we present here a simple and fast volumetric method to inspect differences in vascularization between experimental conditions related to the stimulation and inhibition of CAM angiogenesis based on the Positive Pixel Count algorithm embedded in the APERIO ImageScope software.
PY  - 2015
DO  - 10.1387/ijdb.150025dr
VL  - 59
IS  - 4-5-6
SP  - 217
EP  - 219
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/150025dr
Y2  - 2024/05/08/00:07:52
ER  -