TY  - JOUR
TI  - 2P1, a novel male mouse cDNA specifically expressed during meiosis.
AU  - Hammami-Hamza, Sonia
AU  - Doussau, Mireille
AU  - Allemand, Isabelle
AU  - Segretain, Dominique
AU  - Gasc, Jean-Marie
AU  - Finaz, Catherine
T2  - The International Journal of Developmental Biology
AB  - We screened a mouse germinal cell expression library with a probe derived from Sob1, a human testis-specific cDNA, and identified 2P1, a new mouse cDNA. A database search revealed that 2P1 was 91% identical to ORF1 of E3-3, a rat gene probably involved in the regulation of alternative splicing. Sequencing showed that 2P1 has a destabilization motif in its 3'-untranslated region. Northern blotting showed strong gene expression in the testis and weak expression in the epididymis, with no signal detected in other tissues. RT-PCR analysis confirmed testis and epididymis expression. In situ hybridization revealed that 2P1 mRNA was absent in spermatogonia but expressed in spermatocytes. This last result was confirmed by RT-PCR of FACS isolated primary spermatocytes (pachytene stage). Using RT-PCR, purified spermatids were also shown to express 2P1.
PY  - 2003
VL  - 47
IS  - 1
SP  - 71
EP  - 76
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/12653254
Y2  - 2024/05/04/17:51:23
ER  -