TY  - JOUR
TI  - Physiological and induced apoptosis in sea urchin larvae undergoing metamorphosis.
AU  - Roccheri, Maria C
AU  - Tipa, Cinzia
AU  - Bonaventura, Rosa
AU  - Matranga, Valeria
T2  - The International Journal of Developmental Biology
AB  - Paracentrotus lividus embryos at the early pluteus stage undergo spontaneous apoptosis. Using a TUNEL (TdT-mediated dUTP Nick-End Labelling) assay on whole mount embryos, we showed that there was a different distribution of the apoptotic cells in different optical sections. Not more than 20% of cells in plutei were spontaneously apoptotic, as confirmed by the counts of dissociated ectoderm and intestine cells. Observation of larva stages closer to metamorphosis confirmed that apoptosis is a physiological event for the development of the adult. In particular, larvae at different developmental stages showed apoptotic cells in the oral and aboral arms, intestine, ciliary band and both apical and oral ganglia. Moreover, we found that the number of apoptotic cells decreased in later larva stages, possibly because in the organism approaching metamorphosis, a smaller number of cells needs to be eliminated. Furthermore, combined phorbol ester (TPA) and heat shock treatment enhanced apoptosis by increasing the number of cells involved in the phenomenon.
PY  - 2002
VL  - 46
IS  - 6
SP  - 801
EP  - 806
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/12382946
Y2  - 2024/05/02/01:31:35
ER  -