TY  - JOUR
TI  - Differential post-transcriptional regulations of wnt mRNAs upon axolotl meiotic maturation.
AU  - Vaur, Sabine
AU  - Montreau, Nicole
AU  - Dautry, Francois
AU  - Andeol, Yannick
T2  - The International Journal of Developmental Biology
AB  - The products of the Wntgene family play an essential role in several aspects of embryo patterning. We have investigated the post-transcriptional regulation of three of these genes: Awnt-1, Awnt-5A and Awnt-5B during axolotl (Ambystoma mexicanum) oogenesis, oocyte maturation and early development. We show that Awnt-1, Awnt-5A and Awnt-5B mRNAs are maternally expressed. The three transcripts are tightly regulated at specific times and display differential mRNA stability, poly(A) tail length and localization. In contrastto Awnt-5Bwhich is restricted to the animal hemisphere, Awnt-1 and Awnt-5A have no particular localization in stage VI oocytes. Interestingly, these two mRNAs exhibit a polyadenylation gradient along the animal-vegetal axis. Moreover, after meiotic maturation, Awnt-1 and 5A mRNAs become exclusively localized to the animal pole. This isthe first evidence of a complete mRNA re-localization to the animal hemisphere during oocyte maturation.
PY  - 2002
VL  - 46
IS  - 5
SP  - 731
EP  - 739
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/12216985
Y2  - 2024/05/04/14:45:25
ER  -