TY  - JOUR
TI  - Cell cycle phase, cellular Ca2+ and development in Dictyostelium discoideum.
AU  - Azhar, M
AU  - Kennady, P K
AU  - Pande, G
AU  - Espiritu, M
AU  - Holloman, W
AU  - Brazill, D
AU  - Gomer, R H
AU  - Nanjundiah, V
T2  - The International Journal of Developmental Biology
AB  - In Dictyostelium discoideum, the initial differentiation of cells is regulated by the phase of the cell cycle at starvation. Cells in S and early G2 (or with a low DNA content) have relatively high levels of cellular Ca2+ and display a prestalk tendency after starvation, whereas cells in mid to late G2 (or with a high DNA content) have relatively low levels of Ca2+ and display a prespore tendency. We found that there is a correlation between cytosolic Ca2+ and cell cycle phase, with high Ca2+ levels being restricted to cells in the S and early G2 phases. As expected on the basis of this correlation, cell cycle inhibitors influence the proportions of amoebae containing high or low Ca2+. However, it has been reported that in the rtoA mutant, which upon differentiation gives rise to many more stalk cells than spores (compared to the wild type), initial cell-type choice is independent of cell cycle phase at starvation. In contrast to the wild type, a disproportionately large fraction of rtoA amoebae fall into the high Ca2+ class, possibly due to an altered ability of this mutant to transport Ca2+.
PY  - 2001
VL  - 45
IS  - 2
SP  - 405
EP  - 414
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/11330860
Y2  - 2024/05/04/10:20:57
ER  -