TY  - JOUR
TI  - Intraovarian transplantation of stage I-II follicles results in viable zebrafish embryos 
AU  - Csenki, Zsolt
AU  - Zaucker, Andreas
AU  - Kovács, Balázs
AU  - Hadzhiev, Yavor
AU  - Hegyi, Árpád
AU  - Lefler, Katalin-Kinga
AU  - Müller, Tamás
AU  - Kovács, Róbert
AU  - Urbányi, Béla
AU  - Váradi, László
AU  - Müller, Ferenc
T2  - The International Journal of Developmental Biology
AB  - Maternal gene products drive early embryogenesis almost exclusively until the mid blastula transition (MBT) in many animal models including fish. However, the maternal contribution to embryogenesis does not stop at MBT, but continues to be an essential regulator of key developmental processes. The extent to which maternal effects contribute to embryonic and larval development is hard to estimate due to the technical difficulty of interfering with maternal gene products by conventional forward and reverse genetic tools. Therefore, novel methods to manipulate maternal factors in oocytes need to be developed. Here, we provide a proof of principle protocol for transplanting stage I-II zebrafish follicles into recipient mothers where donor stage I oocytes can develop to stage IV in 2 weeks and in 3 weeks they develop into mature eggs and produce viable offspring. Moreover, we show that simple microinjection of stage I-II follicles with RNA results in reporter gene expression in oocytes and paves the way for developing tools for interfering with maternal gene activity. This early stage oocyte transplantation protocol provides a means to study cellular and molecular aspects of oocyte development in the zebrafish. 
PY  - 2010
DO  - 10.1387/ijdb.082786zc
VL  - 54
IS  - 4
SP  - 585
EP  - 589
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/082786zc
Y2  - 2024/05/02/00:52:02
ER  -