TY  - JOUR
TI  - Prolonged human/sheep cellular chimerism following transplantation of human hemopoietic stem cells
into the ewe celomic cavity
AU  - Michelini, Monica
AU  - Papini, Sandra
AU  - Rosellini, Alfredo
AU  - Noia, Giuseppe
AU  - Ligato, MariaS.
AU  - Mancuso, Salvatore
AU  - Cavazzana, Andrea
AU  - Bertacca, Gloria
AU  - Di Cristofano, Claudio
AU  - Saccardi, Riccardo
AU  - Urbani, Serena
AU  - Revoltella, RobertoP.
T2  - The International Journal of Developmental Biology
AB  - We evaluated the possibility of prolonged chimerism formation in fetus and lamb, following human cord blood-selected CD133+ hemopoietic stem cell (HSC) transplantation into the celomic cavity of ewes at a pre-immune fetal age (44-45 days of pregnancy). Nineteen ewes were injected with HSC and 5 controls with a saline solution. By PCR, HLA-DQalpha1 and 6 human microsatellites (CODIS) were used for HSC traceability. FISH analysis was performed with 8 human DNA probes from different chromosomes, to confirm chromosomal integrity, nuclear DNA localization and donor DNA identification. Immunological staining for revealing HLA-DQalpha1 expression demonstrated multilineage engraftment. Both HLA-DQalpha1 and microsatellites were detected in different tissues of 3 available aborted fetuses, to a lesser extent in 11 lambs tested at 2-months, but not 12-months after birth. Although only 1 fetus of siblings of each sheep was injected, all siblings revealed positive engraftments. Microsatellite analysis showed evidence of human allele segregation in different tissues of individual fetuses and lambs. FISH analysis confirmed chimerism and the presence of human chromosomes. Non-detection of some human gene sequences in different chromosomes and random finding of allele segregation for some human heterozygous microsatellites were found in different tissues of individual animals. Controls born from un-transplanted ewes never revealed any human DNA sequences nor HLADQalpha1 expression.
PY  - 2008
DO  - 10.1387/ijdb.072362mm
VL  - 52
IS  - 4
SP  - 365
EP  - 370
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/072362mm
Y2  - 2024/05/06/15:02:20
ER  -