TY  - JOUR
TI  - The formation of primordial germ cells from germline cells in spherical embryos derived from the blastodisc of 2-cell embryos in goldfish, <I>Carassius auratus</I>
AU  - Otani, Satoshi
AU  - Kitauchi, Tomoe
AU  - Saito, Taiju
AU  - Sakao, Suzu
AU  - Maegawa, Shingo
AU  - Inoue, Kunio
AU  - Arai, Katsutoshi
AU  - Yamaha, Etsuro
T2  - The International Journal of Developmental Biology
AB  - The property of primordial germ cells (PGCs) in fragmented goldfish embryos was investigated. When 1- and 2- cell embryos were cut at several perpendicular levels at the animal-vegetal axis, cells expressing vas  mRNA were observed in the resultant embryos derived from all kinds of animal fragments. Blastodisc fragments from the 1- to 2-cell stage developed to spherical embryos containing yolk body with a yolk syncytial layer (YSL). Germ ring and no tail  expression were not observed in the spherical embryo. When the spherical embryo labeled with tracer dye or GFP-nos1  3'UTR mRNA was transplanted onto the animal part of the blastoderm in a host embryo at the blastula stage, PGCs of spherical embryo origin were detected around the gonadal ridges in the resultant embryos which developed normally. These results suggest that small animal fragments should contain factors sufficient for PGC differentiation and that PGCs differentiate without mesoderm induction, since mesoderm is not induced in a spherical embryo.
PY  - 2005
DO  - 10.1387/ijdb.052027so
VL  - 49
IS  - 7
SP  - 843
EP  - 850
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/052027so
Y2  - 2024/04/29/09:38:35
ER  -